The principle and the future trends of loop-mediated isothermal amplification (LAMP) method
نویسندگان
چکیده
منابع مشابه
Determination of Herpetic Keratitis Frequency Using Loop Mediated Isothermal Amplification (LAMP) Method in Isfahan, Iran
Herpetic keratitis which is resulted from herpes simplex virus type I, is considered the most common cause of corneal blindness that needs being diagnosed and treated in order to prevent irreversible eye effects. The best method to diagnose it is molecular method which has high sensitivity and exclusivity. The current study was done on 25 eye samples collected from patients referred to the Feyz...
متن کاملDetermination of Herpetic Keratitis Frequency Using Loop Mediated Isothermal Amplification (LAMP) Method in Isfahan, Iran
Herpetic keratitis which is resulted from herpes simplex virus type I, is considered the most common cause of corneal blindness that needs being diagnosed and treated in order to prevent irreversible eye effects. The best method to diagnose it is molecular method which has high sensitivity and exclusivity. The current study was done on 25 eye samples collected from patients referred to the Feyz...
متن کاملElectric LAMP: Virtual Loop-Mediated Isothermal AMPlification
We present eLAMP, a PERL script, with Tk graphical interface, that electronically simulates Loop-mediated AMPlification (LAMP) allowing users to efficiently test putative LAMP primers on a set of target sequences. eLAMP can match primers to templates using either exact (via builtin PERL regular expressions) or approximate matching (via the tre-agrep library). Performance was tested on 40 whole ...
متن کاملLoop-Mediated Isothermal Amplification (LAMP) for the Rapid Diagnosis of Herpes Simplex Virus Type 1 (HSV-1)
Background and Aims: considering difficulties in usual laboratory methods in detection of viral infections, improved DNA-based diagnostic techniques are more reliable. Loop mediated isothermal amplification method (LAMP) is a nucleic acid amplification method that amplifies DNA using six primers which has been developed to diagnose viruses as a rapid and high efficiency test. In this study, the...
متن کاملتشخیص بیماریها با استفاده از (Loop-Mediated Isothermal Amplification (LAMP در دامپزشکی
مقدمه و هدف: نیاز به روشی ساده تر، با کارایی بالاتر، سریعتر و ارزانتر نسبت به روشهای موجود جهت تشخیص بیماریهای دامپزشکی روش واکنش زنجیره ای پلی مراز (PCR) ، روشی سریع و حساس می باشد که قادر است تعداد نسخه اندکی از DNA هدف را تا سطحی که توسط ژل الکترفورز قابل تشخیص باشد، تکثیر نماید.بعلت کارایی بالا و قدرت زیاد PCR ، این روش در سالهای اخیر بطور گسترده ای در تشخیص پاتوژن های مختلف بیماریزا اعم ا...
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ژورنال
عنوان ژورنال: NIPPON SUISAN GAKKAISHI
سال: 2007
ISSN: 1349-998X,0021-5392
DOI: 10.2331/suisan.73.296